Microalgae to Bioenergy: Optimization of Aurantiochytrium sp. Saccharification

Microalgae are a promising feedstock for bioethanol production, essentially due to their high growth rates and absence of lignin. Hydrolysis—where the monosaccharides released further fermentation—is considered critical step, its optimization is advised each raw material. The present study focuses on thermal acid hydrolysis (with sulfuric acid) Aurantiochytrium sp. through response surface methodology (RSM), studying effect concentration, time biomass/acid ratio both sugar concentration hydrolysate biomass conversion yield. Preliminary studies allowed establish range variables be optimized. obtained models predicted maximum (18.05 g/L; R2 = 0.990) after 90 min hydrolysis, using 15% (w/v) at 3.5% (v/v), whereas yield (12.86 g/100 g; 0.876) was 9.3% ratio, maintaining other parameters. Model outputs indicate that most influential parameters models, respectively. obtain predictive model very well adjusted experimental data find best saccharification conditions microalgae.